Wednesday, March 12, 2014

LON1 and dimorphic switching caused by gluten or casein? Do 4 different types of infection have Lon? thus 4 types of gluten sensitivity

This post of the blog is going to focus on the LON1 enzyme which in e.coli causes a change in morphology : rod to string (lon stands for LONG ) I am looking for the same change in the other infections I believe to be gluten sensitive.

This page should compliment the 4 types of gluten post if we postulate that gluten and casein act on Lon to change the morphology.

Test of Lon came up negative. It is not gluten sensitive.  Looking at a similar serine protease that is also casein sensitive but when i looked at the list of infections that are gluten sensitive 2 really don't fit t.gondii and strep but all of these infections are "intestine and BBB crossers" so the new theory is Gluten and/or casein merely increase inflammation.  

1. E.coli
In E.coli LON1 can be controlled by casein
REF to come
http://www.ncbi.nlm.nih.gov/pubmed/24520911 (casein acting on Lon)
http://www.ncbi.nlm.nih.gov/pubmed/11045626  (casein as a stimulator acting on Lon)
decided to add paper link  http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2144696/
http://www.ncbi.nlm.nih.gov/pubmed/10734549
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC216189/ 
http://www.ncbi.nlm.nih.gov/pubmed/3042779 (sequence)

In E.coli does gluten based on a similar sequence do the same thing?

2. sutterella
Lon and other Bacterias
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3492299/
Can we find Lon in Sutterella which the the bacteria associated with autism?
Sutterella does have a long form.

3. Fungal infections
In Candida which can change morphology, mold to yeast, is the LON enzyme involved ?
Here are papers where compounds appear to effect the morphology of fungus:
REF to come?

Lon and Mdj1 sequences are homologous among dimorphic fungi
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1405898/
http://www.ncbi.nlm.nih.gov/pubmed/11447604

Paracoccidioides brasiliensis where LON seems to be involved with the dimorphic switching between mold and yeast when the temperature changes
http://www.ncbi.nlm.nih.gov/pubmed/17166750

In Candida does a sequence in corn gluten act like gluten and casein on LON?
Ref
http://www.ncbi.nlm.nih.gov/pubmed/22693589

some strains of candida change morphology with temperature
http://www.ncbi.nlm.nih.gov/pubmed/6998950

4.Mycobacteria
Mycobacterias and LON (they must change morphology too....and will be gluten and casein sensitive)
http://www.ncbi.nlm.nih.gov/pubmed/9425059
http://www.ncbi.nlm.nih.gov/pubmed/11045626 (casein acting on a mycobacteria Lon)

There are rough and smooth morphologies for mycobacteria.
http://www.ncbi.nlm.nih.gov/pubmed/13894939?dopt=Abstract&holding=f1000,f1000m,isrctn

Useful INFO:
hmm....Lon differences?  http://www.ncbi.nlm.nih.gov/pubmed/22119779 
PinA inhibits only the protease binding site http://jb.asm.org/content/170/7/3016.full.pdf
note that Lon was the first atp-dependent protease discovered 

 I need to find the stimulating sequence of casein that acts on Lon and see if it matches gluten...what i have stumbled across seems to have brought me back to where i started. (not the protease site)

I had read about organic crab grass prevention using corn gluten by ohio state.
Corn gluten haults the roots of grass seed from forming....here now... I found a paper where in plants Lon mutations hurt shoots and roots: http://www.ncbi.nlm.nih.gov/pubmed/22968828

Can anyone out there follow my logic?

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